Lab Report Practice (September 11, 2014)
Abstract
The basic organic compounds in every human being are essential to life. When testing for each one, we observed color changes along with other basic observations. We used correct lab techniques to ensure no data was skewed or contaminated.
Introduction
Before performing the lab, we explored the basic compounds necessary for life and found that these include carbohydrates, lipids, proteins, and nucleic acids. These compounds are used in a process called metabolism to synthesize structures like nervous tissue and break down certain substances for energy. It’s important to understand the purpose of each of these compounds, and how they contribute as building blocks to form more complex molecules and polymers. In our experiment, we wanted to test to see if the organic compounds of the carbohydrates, proteins, and lipids matched the labeled nutrition of both the lemonade and the sprite. In order to find out whether these organic compounds existed in both drinks, indicator would be used to detect the presence of any these organic compounds. Carbohydrates include the sugars in the organic compounds. These sugars are formed of monomers known as monosaccharides and include glucose, fructose, and galactose. Proteins are made up of monomers called amino acids that regulate chemical reactions. Finally, lipids are used for energy storage in organism and carry chemical chemical messages. We hypothesize that the labels are accurate as to the organic compounds contained in the each drink.
Methods
This study was conducted at New Tech High School, in Kim Wootton’s Biology classroom, on September 9th, 2014. We tested for four different compounds: starches, lipids, proteins, and sugar. Using different control groups for each individual experiment, we started each experiment off with distilled water.
For the simple sugar test, the first of four tests, we used two test tubes with five drops of water in one of them and five drops of glucose in another; we also added five drops of Benedict’s solution into each tube. Then we continued by placing both test tubes in a beaker full of boiling distilled water.
The second experiment, the starch test, included two test tubes with 5 drops of water in one tube and 5 drops of starch solution in the other. Preceding the initial steps, we added a single drop of iodine into both test tubes.
The protein test, the third test, involved two test tubes, with ten drops of water in one and ten drops of gelatin in another. We then added three drops of CuSO4 into both tubes and thirty drops of NAOH into both as well.
The lipid test, the final test, takes the most amount of time to complete because of the wait time for results. We spread 2 drops of oil onto a piece of brown paper next to two drops of water.
Custom Experiment
The second custom lab was conducted at New Tech High School, in Kim Wootton’s Biology classroom, on September 11th, 2014. For this experiment we tested two liquids for simple sugars, starches, lipids, and protein. Since we had our data from the previous experiment we did not have to have a control group. The two liquids that we tested were sprite and lemonade.
First, we tested for simple sugars by putting five drops of each liquid into a two test tubes. Then we added five drops of Benedict’s solution into the test tubes. Finally we placed both test tubes in boiling distilled water.
Our second experiment tested for starch which included putting five drops of each liquid into test tubes and then adding a single drop of iodine.
The third experiment we tested for protein by putting ten drops of sprite and lemonade into two test tubes. We then added three drops of CuSO4 and thirty drops of NAOH.
For our final experiment we tested for lipids in our two liquid. We tested this by putting two drops of each liquid on a paper towel, and observing the change over time.
Results
After completing all four components of this lab, we got solid results for each organic compound in each substance.
In the first test, although the initial colors in both test tubes were a light blue, after placing both test tubes into the boiling water, the tube that contained the glucose in it changed to a dark orange color while the other tube remained the light blue color.
After the second test, we found that the tube with the starch solution turned a purple color towards the bottom of the tube, proving in fact that the tube with the solution other than water was in fact starch.
The third test, the protein test, had the most change out of any of the tests. While all of the other tests only had one solution change, both solutions changed color in this test. The test tube that contained water, turned to the color of the copper sulfate once we added the thirty drops of NAOH. When we added the thirty drops of NAOH to the gelatin solution, it had a more drastic change. It changed to a dark purple color. Although both solutions changed colors, we can conclude that the gelatin solution contained the protein.
During the wait for the lipid test results, we saw that the oil stained the brown paper whereas the water eventually dried up. Because of these findings, the mystery solution was in fact oil (a lipid).
Custom Experiment Results
Once we completed the experiment we got plausible results for both sprite and lemonade.
The first experiment tested for simple sugars, we found that both sprite and lemonade had simple sugars because once we heated them up we found the both turned a dark shade of orange determining that they test positive for sugar.
Our second experiment tested for starch, we found that the test tube with the lemonade turned green meaning that it tested negative for starch. While our test tube that contained sprite turned a dark blue indicating that there was starch in it.
For our third experiment we tested for protein, once we finished adding thirty drops of NAOH to both test tubes we notice the color changed from a light color to dark blue in both liquids. The dark blue indicated that it tested negative for both, so no protein was in either lemonade or sprite.
The final experiment was testing for lipids. The test for lipids was negative for both sprite and lemonade. We came to this conclusion when we observed that both spots on the paper towel dried up without leaving a mark. If there were lipids in the liquids the paper towel would have been translucent in the spot the liquids evaporated.
Discussion
While one drink is a powdered mix, and the other, a pre bottled drink, our experiment revealed that both drinks tested positive and negative in relation to each respective drink label. The lemonade had a color change for sugar, but tested negative for lipids, and proteins. As for the sprite, the indicator changed color for sugar and starch, and tested negative for protein and lipids.
Conclusion
The purpose of the organic compounds lab was to learn about different common organic compounds and how to test what kind it can be. We now know how to test different solutions for evidence of protein, sugar, lipid and starch. Also the labels of each drink, regardless of pre-bottled or powder-based, follows its nutrition label, and does not contain any discrepancies
Sources Cited:
NC State University. (2004). SAMPLE LAB REPORT. Retrieved from http://www.ncsu.edu/labwrite/res/labreport/res-sample-labrep1.html
Wootton, K. (2014). Organic Compounds Investigation and Notes. Retrieved from https://docs.google.com/a/newtech.coppellisd.com/viewer?url=https://echo.newtechnetwork.org/sites/default/files/new_uploads/20140911/_1410456623_Coacervate%2520Formation%2520Lab.doc
Wootton, K. (2014). Organic Compounds Investigation and Notes. Retrieved from https://docs.google.com/a/newtech.coppellisd.com/viewer?url=https://echo.newtechnetwork.org/sites/default/files/new_uploads/20140909/_1410274612_Organic%2520Compounds%2520Investigation%2520and%2520Notes-2.docx
Abstract
The basic organic compounds in every human being are essential to life. When testing for each one, we observed color changes along with other basic observations. We used correct lab techniques to ensure no data was skewed or contaminated.
Introduction
Before performing the lab, we explored the basic compounds necessary for life and found that these include carbohydrates, lipids, proteins, and nucleic acids. Organic chemistry molecules are functional groups and are very important. These compounds are used in a process called metabolism to synthesize structures like nervous tissue and break down certain substances for energy. It’s important to understand the purpose of each of these compounds, and how they contribute as building blocks to form more complex molecules and polymers. In our experiment, we wanted to test to see if the organic compounds of the carbohydrates, proteins, and lipids matched the labeled nutrition of both the lemonade and the sprite. In order to find out whether these organic compounds existed in both drinks, indicator would be used to detect the presence of any these organic compounds. Carbohydrates include the sugars in the organic compounds. These sugars are formed of monomers known as monosaccharides and include glucose, fructose, and galactose. Proteins are made up of monomers called amino acids that regulate chemical reactions. Finally, lipids are used for energy storage in organism and carry chemical chemical messages. We hypothesize that the labels are accurate as to the organic compounds contained in the each drink.
Methods
This study was conducted at New Tech High School, in Kim Wootton’s Biology classroom, on September 9th, 2014. We tested for four different compounds: starches, lipids, proteins, and sugar. Using different control groups for each individual experiment, we started each experiment off with distilled water.
For the simple sugar test, the first of four tests, we used two test tubes with five drops of water in one of them and five drops of glucose in another; we also added five drops of Benedict’s solution into each tube. Then we continued by placing both test tubes in a beaker full of boiling distilled water.
The second experiment, the starch test, included two test tubes with 5 drops of water in one tube and 5 drops of starch solution in the other. Preceding the initial steps, we added a single drop of iodine into both test tubes.
The protein test, the third test, involved two test tubes, with ten drops of water in one and ten drops of gelatin in another. We then added three drops of CuSO4 into both tubes and thirty drops of NAOH into both as well.
The lipid test, the final test, takes the most amount of time to complete because of the wait time for results. We spread 2 drops of oil onto a piece of brown paper next to two drops of water.
Custom Experiment
The second custom lab was conducted at New Tech High School, in Kim Wootton’s Biology classroom, on September 11th, 2014. For this experiment we tested two liquids for simple sugars, starches, lipids, and protein. Since we had our data from the previous experiment we did not have to have a control group. The two liquids that we tested were sprite and lemonade.
First, we tested for simple sugars by putting five drops of each liquid into a two test tubes. Then we added five drops of Benedict’s solution into the test tubes. Finally we placed both test tubes in boiling distilled water.
Our second experiment tested for starch which included putting five drops of each liquid into test tubes and then adding a single drop of iodine.
The third experiment we tested for protein by putting ten drops of sprite and lemonade into two test tubes. We then added three drops of CuSO4 and thirty drops of NAOH.
For our final experiment we tested for lipids in our two liquid. We tested this by putting two drops of each liquid on a paper towel, and observing the change over time.
Results
After completing all four components of this lab, we got solid results for each organic compound in each substance.
In the first test, although the initial colors in both test tubes were a light blue, after placing both test tubes into the boiling water, the tube that contained the glucose in it changed to a dark orange color while the other tube remained the light blue color.
After the second test, we found that the tube with the starch solution turned a purple color towards the bottom of the tube, proving in fact that the tube with the solution other than water was in fact starch.
The third test, the protein test, had the most change out of any of the tests. While all of the other tests only had one solution change, both solutions changed color in this test. The test tube that contained water, turned to the color of the copper sulfate once we added the thirty drops of NAOH. When we added the thirty drops of NAOH to the gelatin solution, it had a more drastic change. It changed to a dark purple color. Although both solutions changed colors, we can conclude that the gelatin solution contained the protein.
During the wait for the lipid test results, we saw that the oil stained the brown paper whereas the water eventually dried up. Because of these findings, the mystery solution was in fact oil (a lipid).
Custom Experiment Results
Once we completed the experiment we got plausible results for both sprite and lemonade.
The first experiment tested for simple sugars, we found that both sprite and lemonade had simple sugars because once we heated them up we found the both turned a dark shade of orange determining that they test positive for sugar.
Our second experiment tested for starch, we found that the test tube with the lemonade turned green meaning that it tested negative for starch. While our test tube that contained sprite turned a dark blue indicating that there was starch in it.
For our third experiment we tested for protein, once we finished adding thirty drops of NAOH to both test tubes we notice the color changed from a light color to dark blue in both liquids. The dark blue indicated that it tested negative for both, so no protein was in either lemonade or sprite.
The final experiment was testing for lipids. The test for lipids was negative for both sprite and lemonade. We came to this conclusion when we observed that both spots on the paper towel dried up without leaving a mark. If there were lipids in the liquids the paper towel would have been translucent in the spot the liquids evaporated.
Discussion
While one drink is a powdered mix, and the other, a pre bottled drink, our experiment revealed that both drinks tested positive and negative in relation to each respective drink label. The lemonade had a color change for sugar, but tested negative for lipids, and proteins. As for the sprite, the indicator changed color for sugar and starch, and tested negative for protein and lipids.
Conclusion
The purpose of the organic compounds lab was to learn about different common organic compounds and how to test what kind it can be. We now know how to test different solutions for evidence of protein, sugar, lipid and starch. Also the labels of each drink, regardless of pre-bottled or powder-based, follows its nutrition label, and does not contain any discrepancies
Sources Cited:
NC State University. (2004). SAMPLE LAB REPORT. Retrieved from http://www.ncsu.edu/labwrite/res/labreport/res-sample-labrep1.html
Wootton, K. (2014). Organic Compounds Investigation and Notes. Retrieved from https://docs.google.com/a/newtech.coppellisd.com/viewer?url=https://echo.newtechnetwork.org/sites/default/files/new_uploads/20140911/_1410456623_Coacervate%2520Formation%2520Lab.doc
Wootton, K. (2014). Organic Compounds Investigation and Notes. Retrieved from https://docs.google.com/a/newtech.coppellisd.com/viewer?url=https://echo.newtechnetwork.org/sites/default/files/new_uploads/20140909/_1410274612_Organic%2520Compounds%2520Investigation%2520and%2520Notes-2.docx